In late June 2026, Cara and Perry (both natives of Philadelphia) attended PhysFest 5 at the University of Pennsylvania, the fifth installment of a hands-on plant physiology workshop designed to train roughly 40 grad students in key ecophysiology measurements. The workshop offered four full days of lectures and instruction on instruments related to plant water relations, thermal and hyperspectral imaging, environmental sensors, and gas exchange and fluorescence. In the final two days of the workshop, Cara and Perry teamed up with five other PhysFest attendees from Columbia, Oregon State, University of Florida, and University of Utah to craft a project idea that would be carried out in the nearby Woodlands Cemetery. Perry thought it could be interesting to use the instruments at PhysFest to assess how nighttime light pollution might affect leaf physiology. To test this, the group chose to put the Specim IQ Hyperspectral Camera and the LICOR LI-6800 gas exchange system to work in the cemetery. Given the ghostly slant to their sampling environment and the use of leaf spectral data, the group aptly named themselves the Woodlands Cemetery SPECtres. The group got to work looking for the right tree to sample, and found a beech tree with the characteristics they were looking for: one portion of the canopy directly next to a streetlight illuminated throughout the night, another portion away from the streetlight and shaded during the day, and another portion away from the streetlight but fully sunlit during the day. After collecting measurements on the three leaf types, the SPECtres found that the near-infrared (NIR) reflectance of both daytime-shaded leaves was about twice that of the sunlit leaf, but there was little difference in NIR reflectance between the daytime-shade/nighttime-lit and daytime-shade/nighttime-shade leaves. They then made photosynthetic light response curves for all leaves. As expected, the sun-adapted leaf had the highest saturation rate of CO2 assimilation. The daytime-shade/nighttime-lit leaf, however, had the lowest assimilation rate across light conditions, and the daytime-shade/nighttime-shade had intermediate assimilation, meaning exposure to nighttime artificial light could potentially decrease photosynthetic efficiency. Each of the six groups presented their projects at the end of the course to a panel of judges (the PhysFest instructors) in founding-father-esque powdered wigs. After a long week of learning, Perry and Cara led most of the cohort on a Philly tourist sightsee and bar crawl. They felt good being back in Philadelphia and were grateful to take new skills and friendships away from it.

















